Collect. Czech. Chem. Commun. 2009, 74, 443-452
Published online 2009-03-06 09:32:19

Voltammetric behavior of 4-acetamidohippuric acid and 4-acetamidobenzoic acid on a disposable carbon electrode and their determination in human urine

Lai-Hao Wang* and Shih-Ying Zeng

Department of Applied Chemistry, Chia Nan University of Pharmacy and Science, Tainan, Taiwan 71743, Republic of China


The voltammetric behavior of 4-aminobenzoic acid (PABA) and its acetylated metabolite on glassy carbon, carbon fiber or carbon paste electrodes was investigated in an aqueous supporting electrolyte. 4-Aminohippuric acid (4-AHA), 4-acetamidobenzoic acid (4-AMB) and 4-acetamidohippuric acid (4-Ac-AHA) can be separated on a capillary carbon paste electrode in 0.1 M lithium perchlorate. The oxidation potentials of PABA, 4-AHA, 4-AMB and 4-Ac-AHA were 0.70, 0.88, 1.06 and 1.10 V on capillary CPE, respectively. The electrooxidation process is used for simultaneous quantitative determination of acetylated metabolites in urine.

Keywords: 4-Acetamidohippuric acid; 4-Acetamidobenzoic acid; Carbon electrodes; Cyclic voltammetry; Differential pulse voltammetry; Electrochemistry.

References: 20 live references.