Collect. Czech. Chem. Commun. 2003, 68, 2105-2119

Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag for Fluorescence Correlation Spectroscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles. Spectroscopic Behavior in Aqueous Media

Jana Humpolíčkováa, Karel Procházkaa,* and Martin Hofb

a Department of Physical and Macromolecular Chemistry and Laboratory of Specialty Polymers, Faculty of Science, Charles University, Albertov 6, 128 43 Prague 2, Czech Republic
b J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic and Center for Complex Molecular Systems and Biomacromolecules, Dolejškova 3, 182 23 Prague 8, Czech Republic


Fluorescence behavior of octadecylrhodamine B (ORB) and the association-dependent changes in UV-VIS absorption and fluorescence spectra in aqueous solutions were studied by UV-VIS absorption spectroscopy and steady-state and time-resolved fluorometry. Spectral changes are interpreted in terms of the hydrophobically driven formation of H- and J-dimers and higher aggregates. The knowledge gained serves as a basis for the interpretation of results of the fluorescence correlation spectroscopy study of polymeric micelles labeled with ORB.

Keywords: Octadecylrhodamine B-, H- and J-aggregates; UV-VIS absorption; Steady-state and time-resolved fluorometry; Fluorescence correlation spectroscopy; Micelles; Polymers; Dyes; Colloids.

References: 38 live references.