Collect. Czech. Chem. Commun. 1981, 46, 2871-2876

Continuous determination of phenol, vitamin C, lysine and glucose in flowing solutions by means of an amperometric enzyme electrode

Lumír Macholána, Petr Londýna and Jiří Fischerb

a Department of Biochemistry, Purkyně University, 611 37 Brno
b Research Institute of Pure Chemicals, Lachema, 621 33 Brno


A model apparatus is described for the continuous determination of compounds susceptible to catalytic oxidation by enzymes attached chemically to a membrane covering Clark's oxygen cell. The substrate concentration is estimated indirectly from the consumption of oxygen, which is proportional to the area under the curve registered at a constant potential of -0.65 V. A linear relation was found for phenol, ascorbic acid, L-lysine, glucose and polyamines. It is possible to analyze 15-30 samples in 1 h, depending on the flow rate of the carrier buffer.