Collect. Czech. Chem. Commun. 1980, 45, 2583-2586

Study of hemoglobin stabilization during lyophilization with saccharides

Tomáš I. Přistoupil, Stanislav Ulrych and Marie Kramlová

Institute of Haematology and Blood Transfusion, 128 20 Prague 2


Isotonic solutions of 6% hemoglobin were lyophilized with 2-10% glucose, sucrose and dextran and were stored at different conditions for periods up to one year. The lyophilizates dissolved in water were analyzed for their content of methemoglobin and of precipitate, as for the absorption spectra and oxyhemoglobin dissociation curves. Sucrose was found to have a significantly higher effect than glucose in stabilizing the original properties of the hemoglobin samples especially during long time storage. The effect of dextran was negligible.