Collect. Czech. Chem. Commun.
1980, 45, 2572-2582
https://doi.org/10.1135/cccc19802572
Hog pancreatic α-amylase peptides from tryptic digest of isozyme AII
Bedřich Meloun, Ivan Kluh and Ladislav Morávek
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
Abstract
Peptides isolated from the tryptic digest of the S-sulfo derivative of isozyme AII by gel filtration on Sephadex columns, paper chromatography and electrophoresis were sequenced by the phenylisothiocyanate method. The sequential regions determined account for 319 nonoverlapping amino acid residues in 46 peptides. Sequences around methionine residues and problems of isolation of amylase isozymes are discussed.
