Collect. Czech. Chem. Commun. 2009, 74, 1351-1360
Published online 2009-09-11 22:43:48

Synthesis of Mn3O4 nanoparticles and their application to cancer cells

Marijan Gotić*, Siniša Ivanković, Svetozar Musić and Tatjana Prebeg

Ruđer Bošković Institute, Bijenička c. 54, HR-10002 Zagreb, Croatia


The Mn3O4 nanoparticles were synthesized by optimizing the precipitation system MnCl2/H2O2/NH4OH. The material thus obtained was microstructurally characterized using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). The Mn3O4 nanoparticles were applied to fibroblast and cancer cells. In the concentration range 25–100 μg/ml the particles showed low cytotoxic effects on L929 fibroblast cells, medium cytotoxic effects on FsaR fibrosarcoma cells and high cytotoxic effects on SCCVII carcinoma cells. N-Acetyl-L-cysteine (NAC) increased cytotoxicity of Mn3O4 nanoparticles, which suggested that reactive oxygen species (ROS) were not responsible for their selective cytotoxic effect. It was shown that the Mn3O4 nanoparticles entered the cancer cells and aggregated inside the membrane-bound spaces that were recognized as destroyed mitochondria.

Keywords: Mn3O4; Hausmannite; Cytotoxicity; Cancer cells; Nanomaterials; Electron microscopy.

References: 30 live references.