Collect. Czech. Chem. Commun. 2007, 72, 996-1004

Synthesis of Novel Coumarin-Based Fluorescent Probes

Ivana Kosiova* and Pavol Kois

Department of Organic Chemistry, Faculty of Natural Sciences, Comenius University, Mlynska dolina, Pavilon CH2, SK-84215 Bratislava, Slovak Republic


We report on synthesis of new fluorescent probes suitable for site-specific incorporation into oligonucleotides. Coumarin derivatives were used as sensitive fluorescent labels and were attached to glycerol unit by two types of linkers as potential building blocks for oligonucleotide synthesis. Spectral characteristics of the functionalized coumarin building blocks were measured.

Keywords: Coumarin; Fluorescent labeling; Oligonucleotides; Fluorescent spectroscopy; Click chemistry; Triazoles; Azides.

References: 42 live references.