Collect. Czech. Chem. Commun. 2006, 71, 698-708

Study of a Novel [CuII(TPP)]/Nafion Film Electrode for the Detection of Nitric Oxide Radical (NO)

Ya-Zhen Wanga,b, Wan-Hua Chena and Sheng-Shui hua,b,*

a Department of Chemistry, Wuhan University, Wuhan 430072, P.R. China
b State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, P.R. China


A novel modified platinum electrode coated with copper(II)-5,10,15,20-tetraphenylporphyrin, [CuII(TPP)], has been developed. The electrochemical behavior of NO at the modified electrode was studied by cyclic and differential pulse voltammetries. In a deoxygenated phosphate-buffered saline solution (pH 7.4), the [CuII(TPP)]/Nafion-modified Pt electrode exhibits a strong electrocatalytic effect and stability to oxidation with NO and can be employed as a NO sensor. In this system, the modification film is capable of removing some endogenous anionic electroactive substances in biological samples, such as nitrite and ascorbate. The oxidation currents increase linearly with the concentration of NO in the range 1.8 × 10-7-0.9 × 10-4 M. The detection limit is 2.0 × 10-8 M. The nitric oxide radical, released from sodium nitroprusside (SNP), was also determined using chronoamperometry in the presence of light.

Keywords: Copper(II)-5,10,15,20-tetraphenylporphyrin; Nitric oxide radical; Chemically modified electrodes; Nafion; Sodium nitroprusside; Cyclic voltammetry; Electrochemistry.

References: 18 live references.