Collect. Czech. Chem. Commun. 2002, 67, 302-313

Amperometric Determination of Glucose with a Carbon Paste Biosensor

Klemens Schachla, Emir Turkušićb, Alena Komersovác, Martin Bartošc, Helmut Modereggera, Ivan Švancarac, Hailemichael Alemud, Karel Vytřasc, Maria Jimenez-Castroa and Kurt Kalchera,*

a Institut für Analytische Chemie, Karl-Franzens-Universität Graz, Universitätsplatz 1, A-8010 Graz, Austria
b Department of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 35, 71000 Sarajevo, Bosnia and Herzegovina
c Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, nám. Čs. legií 565, CZ-53210 Pardubice, Czech Republic
d Department of Chemistry, Addis Ababa University, PO BOX 1176, Addis Ababa, Ethiopia


A simple glucose biosensor has been developed by bulk modification of a carbon paste electrode with glucose oxidase as a biocomponent and manganese dioxide as a mediator. The sensor was employed as amperometric detector in a flow-injection system at 21 °C in 0.2 M phosphate buffer (pH 7.5). At an applied working potential of 0.48 V vs Ag/AgCl and a flow rate of 0.2 ml min-1, the sensor exhibited well reproducible amperometric response to glucose. A linear relation between the peak current and the analyte concentration was found between 20 and 500 mg l-1 glucose with a detection limit (3σ) of 10.5 mg l-1 glucose. The sensor can be operated continuously for 12 h without any loss in the signal height and can be used for the determination of glucose in white wine samples.

Keywords: Glucose; Flow-injection analysis; Biosensors; Carbon paste electrodes; Oxidases; Amperometry.

References: 48 live references.