Collect. Czech. Chem. Commun. 1997, 62, 821-828

Transport of Adefovir (PMEA) in Human T-Lymphoblastoid Cells

Lenka Olšanská, Tomas Cihlar, Ivan Votruba* and Antonín Holý

Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic,166 10 Prague 6, Czech Republic


The uptake of [3H]PMEA by human T-lymphoblastoid cells (CCRF-CEM) apparently proceeds by fluid-phase endocytosis. The transport kinetics was shown to be nonconcentrative and nonsaturable. The uptake takes place even at a low temperature (4 °C), is strictly dependent on the intracellular level of ATP, is not substantially affected by cell suspension density and is not competitively inhibited by other PME derivatives.

Keywords: Adefovir; Transport; Human cells.