Collect. Czech. Chem. Commun. 1996, 61, 1823-1830

Immunosorbents for the Preparation of Mycobacterium bovis BCG Antigen

Sabina Krejčíkováa,b, Milan J. Benešc, Eva Wisingerováb and Jaroslava Turkováa,d

a Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 166 10 Prague 6, Czech Republic
b Institute of Hygiene and Epidemiology, 100 42 Prague 10, Czech Republic
c Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic
d Institute of Pathophysiology, 1st Medical Faculty, Charles University, 128 53 Prague 2, Czech Republic


The preparation of immunosorbents for the isolation of Mycobacterium bovis BCG antigen is described. Polyclonal rabbit antibodies are attached both by oriented immobilization following oxidation with periodate to a cellulose and agarose support with hydrazide groups, and directly to agarose activated with cyanogen bromide. The properties of sorbents in relation to the method of preparation and to the support used are compared. The best properties were exhibited by immunosorbent attached to cellulose beads.

Keywords: Immunosorbent; Mycobacterium bovis BCG antigen; Oriented immobilization.