Collect. Czech. Chem. Commun. 1994, 59, 2209-2226

Reverse-Phase High Performance Liquid Chromatography of Metal Chelates of 4-(2-Pyridylazo)resorcinol and 4-(2-Thiazolylazo)resorcinol. Simultaneous Determination of Low Concentrations of Co, Ni and Fe

Josef Doležal and Lumír Sommer

Department of Analytical Chemistry, Masaryk University, 611 37 Brno, Czech Republic


The behaviour of stable and inert metal chelates of 4-(2-pyridylazo)resorcinol and 4-(2-thiazolylazo)resorcinol in RP HPLC and in its ion-pair modification (IP RP HPLC) on Separon SGX RPS silica gel was studied. Good results were obtained by the ion-pair variant in water methanol solutions at pH 7 in the presence of cetyltrimethylammonium bromide. The technique proved to be convenient for the preconcentration, separation and quantitation of low concentrations of Fe, Co, and Ni in waters.