Collect. Czech. Chem. Commun. 1993, 58, 2605-2611

Preparation of Uniform Size Beads with Enhanced Hydrophilicity of Pore Surface, Based on Macrophorous Poly(glycidyl methacrylate-co-ethylene dimethacrylate)

Vladimír Šmigol and František Švec

Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic


Relative hydrophilicity of poly(glyceryl methacrylate-co-ethylene dimethacrylate) beads before and after modification with epichlorohydrin followed by hydrolysis has been studied. The hydrophilicity was determined from the retention of human serum albumin in a column packed with the polymers using various mobile phases. Hydrophilization dramatically decreases the extent of human serum albumin adsorption and provides beads suitable for size-exclusion HPLC of hydrophobic proteins.