Collect. Czech. Chem. Commun. 1993, 58, 2383-2395

The Mobility of Tryptophan and Dansyl Fluorophores in Labelled Poly(N-ethylacrylamide) and Poly(N-ethylmethacrylamide)

František Mikeš, Drahomír Výprachtický and Jan Pecka

Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic


The mobility of tryptophan fluorophore in N-butyl-Nα-acetyltryptophanamide and in side chain of labelled poly(N-ethylacrylamide) and poly(N-ethylmethacrylamide) was investigated by the fluorescence depolarization method. The mobility of the fluorophore in the low-molecular-weight model is much higher than in side chains of the polymers. Different steric hindrance by the polymer backbone can explain the higher mobility of the fluorophore in poly(N-ethylacrylamide) and in poly(N-ethylmethacrylamide). The mobility of 5-dimethylamino-1-naphthalenesulfonamide (dansyl) fluorophore in side chains of labelled poly(N-ethylmethacrylamide) in the high-viscosity range increases with increasing number of methylene groups in side chains. The low-viscosity range, the rate s of conformational changes in short side chains (n = 2 - 7) are approximately constant and significantly decrease in long side chains (n = 10, 12). The drop in the rates is probably due to intramolecular association of the long hydrophobic chains in water.