Collect. Czech. Chem. Commun. 1992, 57, 2174-2180

A Kinetic Method for Determination of the Biosynthetic Activity of Glutamine Synthetase

Vladimír Mikeš

Department of Biochemistry, Masaryk University, 61137 Brno


In the present paper a rapid and simple kinetic method for determination of the biosynthetic activity of glutamine synthetase is described based upon the decomposition of ATP, accompanied with acidification of the slightly buffered medium which can be measured by means of a pH indicator. The method can be used for determination of the enzyme activity in whole permeabilized cells, crude cell extracts, as well as for kinetic studies and studies of the effects of inhibitors on the purified glutamine synthetase.