Collect. Czech. Chem. Commun. 1992, 57, 2166-2173

Preparation and Properties of Three Immunogens with a Spacer on the β-Side of the Steroid Skeleton and the Effect of Immunogen Structure on Specificity of the Obtained Antisera

Alexander Kasala, Richard Hamplb, Zdeněk Putzc, Ladislav Kohouta and Luboslav Stárkab

a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
b Institute of Endocrinology, 116 94 Prague 1
c Institute of Endocrinology, 034 91 Ľubochňa


Three haptenes for the determination of Reichstein's compound S and progesterone were prepared in which hemisuccinyloxy group was attached to the β-side of the steroid skeleton (compounds VII, XIII and XV). These haptenes were bound to BSA and used for preparation of antisera. The specificity of these antisera was compared with that of antisera obtained with usual immunogens.