Collect. Czech. Chem. Commun. 1992, 57, 204-211

Conjugates of Hepatitis B Surface Antigen (14-32)Pre-S2 Region with N-Acetylmuramic Acid, N-Acetylnormuramic Acid, and Their L-Alanyl-D-Isoglutamine Derivatives: Synthesis and Studies on Immunogenicity

Zbigniev Maćkiewicza, Hanna Świderskab, Maria Kalmanowaa, Zygfryd Smiatacza, Adam Nowosławskib and Gotfryd Kupryszewskia

a Institute of Chemistry, University of Gdańsk, 80-952 Gdansk, Poland
b Department of Imunopathology, National Institute of Hygiene, 00-971 Warszawa, Poland


Four novel analogs of hepatitis B virus surface antigen (14-32)Pre-S2 region fragment attached covalently to N-acetylmuramic acid, N-acetylmuramyl-L-alanyl-D-isoglutamine, N-acetylnormuramic acid, and N-acetylnormuramyl-L-alanyl-D-isoglutamine were synthesized by the solid phase method. The ability of analogs to induce cellular and humoral immunity to native HBsAg was tested on rabbits. Cellular immune response occurred in vitro, and HBs antibodies were detected in all immunized animals. No additional adjuvants were used in the tests.