Collect. Czech. Chem. Commun. 1990, 55, 2321-2327

NADH oxidation during peroxidase catalyzed metabolism of carcinogenic non-aminoazo dye 1-phenylazo-2-hydroxynaphthalene (Sudan I)

Marie Stiborov√° and Pavel Anzenbacher

Department of Biochemistry, Charles University, 128 40 Prague 2


The peroxidase catalyzed oxidation of 1-phenylazo-2-hydroxynaphthalene (Sudan I) in the presence of NADH results in oxidation to oxidized NAD+. This oxidation is supposed to be caused by the reduction of reactive Sudan I metabolite(s). Under certain conditions NADH acts as the agent protecting the cellular nucleophilic compounds such as nucleic acids (DNA, tRNA) against modification caused by reactive metabolite(s) formed from carcinogenic Sudan I by the peroxidase/H2O2 system. The results are discussed from the point of view of the physiological significance of NADH in the initiation of chemical carcinogenesis.