Collection of Czechoslovak Chemical Communications - digital archive 

Abstract

The peroxidase catalyzed oxidation of 1-phenylazo-2-hydroxynaphthalene (Sudan I) in the presence of NADH results in oxidation to oxidized NAD⁺. This oxidation is supposed to be caused by the reduction of reactive Sudan I metabolite(s). Under certain conditions NADH acts as the agent protecting the cellular nucleophilic compounds such as nucleic acids (DNA, tRNA) against modification caused by reactive metabolite(s) formed from carcinogenic Sudan I by the peroxidase/H₂O₂ system. The results are discussed from the point of view of the physiological significance of NADH in the initiation of chemical carcinogenesis.