Collect. Czech. Chem. Commun. 1990, 55, 2100-2109

DNA-dependent RNA polymerase mediated formation of methylphosphonyl internucleotide linkage

Květa Horská, Aleš Cvekl, Ivan Rosenberg, Antonín Holý and Karel Šebesta

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


Analogues of ATP modified in the triphosphate moiety by introducing phosphonylmethyl ether residue(s) were used as substrates for bacterial DNA-dependent RNA polymerase. Although the analogues function as poor substrates they enter the initiation as well as the elongation site of the enzyme. The ATP analogue containing the phosphonylmethyl ether group in the α position in the presence of UTP and poly [d(A-T)] gives rise to alternating di- and trinucleotides with one anomalous internucleotide linkage per molecule.