Collect. Czech. Chem. Commun. 1990, 55, 1730-1740

Scale-up and scale-down techniques for fermentations of polyene antibiotics

Petr Ettler

Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4


Our philosophy of successful biotechnology transfer to industrial scale covers the comparison of complex sets of microbiological, analytical and bioengineering data from cultivations in various scales and different geometries of mixing with laboratory findings. In particular, the availability of nutrients to producing microorganism should be understood, therefore for quick scaling-up procedure of polyene antibiotics produced by Streptomyces noursei we recommend to use physiological marker as total dehydrogenase activity determination. The utility of scale-down tests for identification of process fluctuation, validation of new substrate batches and simultaneous control of inoculum quality was proved.