Collect. Czech. Chem. Commun. 1990, 55, 273-281

The quantification of protein bioactivity, Phage T4 lysozymes substituted at residue 86

Marvin Charton

Chemistry Department, School of Liberal Arts and Sciences, Pratt Institute, Brooklyn, New York 11205, U.S.A.


Data taken from the literature for the activity of mutant Phage T4 lysozymes substituted at residue 86 were correlated with the intermolecular force (IMF) equation with very good results. The activity determined was the relative amount of enzyme required to give a cleared area with a radius of 7.00 mm in the lysoplate assay of Becktel and Baase. The best regression equation obtained shows hydrogen bonding to be the major factor in determining activity, with dispersion forces next in importance and steric effects least. No dependence on side chain charge was observed. The results support the utility of the IMF equation as a quantitative model of protein bioactivity.