Collect. Czech. Chem. Commun. 1989, 54, 523-532

Automated chloridite and amidite synthesis of oligodeoxyribonucleotides on a long chain support using amidine protected purine nucleosides

Luboš Arnolda, Zdeněk Točíka, Eliška Bradkováa, Zdeněk Hostomskýb, Václav Pačesb and Jiří Smrta

a Institute of Organic Chemistry and Biochemistry
b Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 166 10 Prague 6


Preparation of solid supports with long aliphatic spacer for the oligonucleotide synthesis is described. Automated synthesis of oligodeoxyribonucleotides using in situ prepared methylchlorophosphite intermediates from amidine protected purine nucleosides is described. Phosphoramidite synthesis of oligodeoxyribonucleotides mediated by 1-methylimidazole trifluoromethanesulfonate is described. Novel isolation of crude oligonucleotides by ethanol precipitation of potassium salts is described.