Collect. Czech. Chem. Commun. 1989, 54, 3011-3024

Study of polymer chain dynamics in solution by time-resolved spectrofluorometry

Vlastimil Fidlera, Stefan Vajdaa, Zuzana Limpouchováa, Jiří Dvořáka, Karel Procházkaa and Bohumil Bednářb

a Department of Physical Chemistry, Charles University, 128 40 Prague 2
b Department of Polymers, Prague Institute of Chemical Technology, 166 28 Prague 6


The methodology of polarization time-resolved fluorometry and interpretation of its results are outlined at a general level, and the measurement on and use of facilities of the Edinburgh Instruments Model 299T apparatus are discussed in detail. The dynamics of conformational changes in chains of poly(methacrylic acid) containing covalently bonded dansyl labels are studied in aqueous solutions at various pH. It is shown that at pH > 6, the shorter effective rational correlation time τr < 2 ns corresponds to the rotation of the free dansyl label about bonds by which it is attached to the polymeric chain; at pH < 4 the longer effective rational correlation time τr = 20-26 ns corresponds to the rotation of the compact spherical formation constituted by a part of the collapsed polymeric chain in which the label is fixed and whose relative molecular mass is approx. 15 000-20 000.