Collect. Czech. Chem. Commun. 1988, 53, 2645-2653

Simultaneous synthesis of sequence-unrelated peptides derived from proteins of human papillomaviruses

Viktor Krchňáka, Josef Vágnera and Ivan Hirschb

a Léčiva-Pharmaceuticals, 143 10 Prague 4
b Department of Experimental Virology, Institute of Sera and Vaccines, 101 03 Prague 10


Amino acid sequences derived from the early (E) and late (L) coding regions of human papillomaviruses (HPV) type 6, 11, and 16 were analysed by means of a computer program for prediction of B-cell determinants. Eight peptides ranging from 9 to 17 amino acids and containing potential B-cell determinants were selected for synthesis. The peptides were synthesized by using a configuration for solid-phase synthesis that enables the simultaneous synthesis of several peptides in flow reactors at low pressure on standard poly(styrene-1% divinylbenzene)resin. Crude peptides were obtained in good yield and purity. They were then further purified by high-performance liquid chromatography for use as solid antigens in site-directed serology.