Collect. Czech. Chem. Commun. 1988, 53, 2637-2644

Synthesis of modified human C-peptide and its fragments

Běla Bendlováa, Michal Leblb, Pavel Štolbaa and Luboslav Stárkaa

a Research Institute of Endocrinology, 116 94 Prague 1
b Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


Syntheses of the modified human C-peptide containing residues suitable for the introduction of the radioactive label (tyrosine) and internal marker for monitoring binding to carrier (norvaline) and five of its fragments are described. The syntheses were performed by solid phase method using either 9-fluorenylmethoxycarbonyl or tert-butyloxycarbonyl protecting groups. The products were purified by gel filtration, ion exchange chromatography and reversed phase HPLC. The reactivity of prepared peptides with antisera was determined and the modified C-peptide was found fully reactive.