Collect. Czech. Chem. Commun. 1988, 53, 186-197

Membrane proton transport mediated by phenylhydrazonopropanedinitriles

Ľubomír Klukaa, Ernest Šturdíka, Štefan Baláža, Dušan Kordíkb, Michal Rosenberga, Marián Antalíkc and Jozef Augustína

a Department of Biochemical Technology Faculty of Chemical Technology, Slovak Institute of Technology, 812 37 Bratislava
b Czechoslovak Institute of Metrology, 825 62 Bratislava
c Institute of Experimental Physics, Slovak Academy of Sciences, 040 11 Košice


Some fundamental physicochemical characteristics as stability in solutions, solubility in various solvents and association constants describing equilibria with protons and potassium ions in aqueous solutions were determined for phenylhydrazonopropanedinitriles (PHPD). The effect of pH and sodium, potassium, calcium, and magnesium cations on the distribution of PHPD were examined in a two-compartment system 1-octanol-water. The transmembrane transfer of protons by PHPD causing a disturbance of the pH-gradient was verified in vitro using a model three-compartment system water-octanol-water, imitating the in vivo intracristal space-inner mitochondrial membrane – matrix system. Transfer of H+ ions mediated by PHPD in the system under study was found to be considerably faster when an exchange with K+ ions (ion-exchanging antiport H+/K+) was possible. A model was described indicating the reality of ion-exchanging antiport H+/Me+ mediated by PHPD on biomembranes which is in line with the chemiosmotic theory.