Collect. Czech. Chem. Commun. 1987, 52, 522-524

Endoprolylpeptidase (EC, improvement of the isolation method

Karel Hauzera, Tomislav Bartha, Karel Jošta, Jana Barthováb and Linda Hauzerováa

a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
b Department of Biochemistry, Faculty of Sciences, Charles University, 127 10 Prague 2


The improvement of the enzyme isolation method consists in the addition of sodium sulphite (5 mmol l-1) used in the isolation procedure instead of the low-molecular thiols used previously, e.g. 2-mercaptoethanol, cystein, dithiothreitol etc. The protective effect against oxidation of the enzyme by aerial oxygen has been retained while, at the same time, autolysis of the enzyme was decreased and the final enzyme preparation exhibited higher values of specific activity.