Collect. Czech. Chem. Commun. 1984, 49, 1835-1845

Primary structure of the N-terminal region (1-95) of thermitase, a thermostable proteinase from Thermoactinomyces vulgaris

Bedřich Melouna, Miroslav Baudyša, Vladimír Kostkaa, Gert Hausdorfb and Wolfgang Ernst Höhneb

a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague, Czechoslovakia
b Institute of Physiological and Biological Chemistry, Humboldt-University, Berlin, G.D.R.


The 95-residue N-terminal amino acid sequence of thermitase, a thermostable serine proteinase from T. vulgaris, is the following: Tyr-Thr-Pro-Asn-Asp-Pro-Tyr-Phe-Ser-Ser-Arg-Gln-Tyr-Gly-Pro-Gln-Lys-Ile-Gln-Ala-Pro-Gln-Ala-Trp-Asp-Ile-Ala-Glu-Gly-Ser-Gly-Ala-Lys-Ile-Ala-Ile-Val-Asp-Thr-Gly-Val-Gln-Ser-Asn-His-Pro-Asp-Leu-Ala-Gly-Lys-Val-Val-Gly-Gly-Trp-Asp-Phe-Val-Asp-Asn-Asp-Ser-Thr-Pro-Gln-Asn-Gly-Asn-Gly-His-Gly-Thr-His-Cys-Ala-Gly-Ile-Ala-Ala-Ala-Val-Thr-Asn-Asn-Ser-Thr-Gly-Ile-Ala-Gly-Thr-Ala-Pro-Lys. The sequential data were obtained by analyses of peptides isolated from the soluble part of the tryptic digest of the N-terminal cyanogen bromide fragment of the enzyme and from the chymotryptic digest of a thermitase fragment obtained by 2-nitro-5-thiocyanobenzoate cleavage of the enzyme at its single cysteine residue.