Collect. Czech. Chem. Commun. 1984, 49, 1827-1834

A physico-chemical study of the interaction between human hemoglobin and benzene

Pavel Sedláček, Zdeněk Pavlíček and Vítěz Kalous

Department of Physical Chemistry, Charles University, 128 40 Prague 2


The interaction between human hemoglobin and benzene produced difference spectra (in the visible region) changing with time. In addition to these changes we studied the dependence of the interaction on temperature and on concentration of the two components. The interaction of hemoglobin and benzene was also followed by means of CD spectra. These spectra in the region 200-260 nm demonstrate that the secondary structure of hemoglobin was not affected by the interaction. By contrast, in the region 270-300 nm the CD spectra show a change in the quaternary structure of hemoglobin, which goes from the R state to the T state, and after a period it returns to the original state.