Collect. Czech. Chem. Commun. 1982, 47, 2561-2567

Utilization of a recyclization reaction for the analysis of the mechanism of enzymic alcohol oxidation

Jan Kovář and Igor Kučera

Department of Biochemistry, Purkyně University, 611 37 Brno


The kinetics of recyclization reactions catalyzed by horse liver alcohol dehydrogenase in the presence of a coenzyme, p-nitrosodimethylaniline, and various alcohols was studied from theoretical and experimental point of view. It was found that p-nitrosodimethylaniline is an oxidized substrate of the alcohol dehydrogenase suitable for testing basic kinetic parameters of various alcohols, especially for checking the kinetic importance of ternary enzyme-NAD-alcohol complexes.