Collect. Czech. Chem. Commun. 1982, 47, 1530-1536

UDP-D-glucose 4-epimerase activity of the tissue culture of white poplars (Populus alba L. var. pyramidalis)

Ladislav Bilisics, Štefan Karácsonyi and Marta Kubačková

Institute of Chemistry, Slovak Academy of Sciences, 809 33 Bratislava


The presence of UDP-D-glucose 4-epimerase (EC in the culture tissue of white poplar was evidenced. As found, the partially purified enzyme preparation contained UDP-D-glucose glucosyltransferase, UDP-D-galactose galactosyltransferase and non-specific enzymes able to cleave the uridine-diphosphate saccharides into the appropriate hexose monophosphates. The activity change of UDP-D-glucose 4-epimerase in tissue culture cells during the growth was in accord with changes in D-galactose content in cell walls and indicated the possibility to regulate the formation of polysaccharides containing D-galactose at the level of production of UDP-D-galactose in cells.