Collect. Czech. Chem. Commun. 1982, 47, 2746-2748

Oxidation mechanism of carcinogenic bis-azo dyestuff Trypan Blue

Miroslav Matrka and Jana Pípalová

Department of Toxicology, Research Institute of Organic Syntheses, 532 18 Pardubice-Rybitví


Oxidation of Trypan Blue with cerium(IV) ion in acid medium gives arenediazonium cation similar to the oxidation product of N,N-dimethyl-4-aminoazobenzene. Quantitative evaluation of the arenediazonium salt formed has been carried out spectrophotometrically after previous C-azo coupling with 2-naphthol.