Collect. Czech. Chem. Commun.
1981, 46, 667-672
https://doi.org/10.1135/cccc19810667
Synthesis of the uracil analog of thuringiensin and its inhibitory effect on DNA-dependent RNA polymerase of Escherichia coli
Ladislav Kalvoda, Květa Horská and Karel Šebesta
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
Abstract
An analog of thuringiensin (II), containing uracil instead of adenine, was synthetized by the method employed for thuringiensin. Similarly to the parent substance this analog inhibits the DNA-dependent RNA polymerase of Escherichia coli, simultaneously competing with the natural substrate, i.e. UTP. The inhibition constant (Ki = 30 μm) shows that the uracil analog of thuringiensin is an inhibitor of RNA polymerase roughly as efficient as thuringiensin.
