Collect. Czech. Chem. Commun. 1981, 46, 673-677

Degree of inhibition of eukaryotic DNA-dependent RNA polymerases by thuringiensin

Květa Horská and Karel Šebesta

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


It was shown in experiments with purified DNA-dependent RNA polymerases from calf thymus that the inhibition of these enzymes caused by thuringiensin is competitive with respect to ATP. Enzyme AI is more sensitive to the inhibitor (Ki = 0.34 μm) than enzyme B (Ki = 1.49 μm).This finding eliminates the discrepancies with respect to this subject which exist in literature.