Collect. Czech. Chem. Commun. 1980, 45, 617-622

Alpha- and beta-amylase immobilized by a covalent binding to hydroxyalkyl methacrylate gels

Karol Babor and Lubomíra Rexová-Benková

Institute of Chemistry, Slovak Academy of Sciences, 809 33 Bratislava


Insoluble active preparation of alpha- and beta-amylase were prepared by a covalent binding of enzymes to hydroxyalkyl methacrylate gel (various kinds of Spheron type) activated with cyanogen bromide. The amount of the bound enzyme, its amylolytic activity on individual substrates, storage and heat stabilities, and pH-optimum were determined.