Collect. Czech. Chem. Commun. 1980, 45, 611-616

Ribonuclease a catalyzed preparative synthesis of dinucleoside monophosphates containing uridine analogues

Antonina P. Kavunenkoa and Antonín Holýb

a Institute of High-molecular Compounds, Academy of Sciences of the USSR, Leningrad, USSR
b Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague, Czechoslovakia


Preparative synthesis of dinucleoside monophosphates, catalyzed by ribonuclease A, is described. Uridine 2',3' -cyclic phosphate was used as a donor, the acceptors being uridine (Ia), N3-methyl-uridine (Ib), 5-methyluridine (Ic), 6-methyluridine (Id), 3-(β-D-ribofuranosyl)uracil (IIa), 1-methyl-3-(β-D-ribofuranosyl)uracil (IIb), 6-azauridine (III) and 6-methyl-2'-deoxyuridine (IV). The obtained compounds of the type UpN (where N is the nucleoside moiety I-IV) were characterized by paper chromatography, electrophoresis and UV-spectra.