Collect. Czech. Chem. Commun. 1979, 44, 3411-3417

Immbilization on cellulose in bead form after periodate oxidation and reductive alkylation

Jaroslava Turkováa, Jiří Vajčnera, Drahomíra Vančurováa and Jiří Štambergb

a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
b Institute of Macromolecular Chemistry, Academy of Sciences, 162 06 Prague 6


The covalent binding of trypsin to cellulose beads after periodate oxidation was examined. The degree of cellulose derivatives solubilization is directly related to cellulose oxidation and increases with the increasing pH of the reaction mixture. The quantity of trypsin immobilized was examined as a function of pH, reaction time, and concentration of native trypsin in the reaction mixture during the coupling reaction. The catalytic activity and the stability of the preparations of immobilized trypsin during continuous hydrolysis of low-molecular-weight and high-molecular-weight substrates was assayed.