Collect. Czech. Chem. Commun. 1979, 44, 3405-3410

Immobilization of trypsin on periodate-oxidized glucosehydroxyalkyl methacrylate gel Separon-H 1 000-glc

Drahomíra Vančurováa, Jaroslava Turkováa, Jiří Čoupekb and Anna Frydrychováb

a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
b Laboratory Instruments Works, 162 03 Prague 6


The dependence of the amount of bound enzyme on the oxidation time, molarity of the oxidizing solution, pH, time, temperature and enzyme concentration in the reaction mixture were investigated for the case where trypsin is covalently bound on the hydroxyalkyl methacrylate gel containing glucose on its surface (Separon-H 1 000-glc) after the gel had been periodate-oxidized. The immobilized derivatives of trypsin were examined with respect to their catalytic activity and stability in continuous hydrolysis.