Collect. Czech. Chem. Commun. 2011, 76, 1991-2004
Published online 2012-01-31 09:50:08

Polarographic and voltammetric determination of genotoxic nitro derivatives of quinoline using mercury electrodes

Vlastimil Vyskočila,*, Ivan Jiráneka, Aleš Daňhela, Jiří Zimaa, Jiří Bareka, Joseph Wangb and Karolina Peckováa

a Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, 128 43 Prague 2, Czech Republic
b Department of Nanoengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0448 La Jolla, CA, USA


Electrochemical behavior of genotoxic nitro derivatives of quinoline, namely 5-nitroquinoline (5-NQ), 6-nitroquinoline (6-NQ) and 8-nitroquinoline (8-NQ), was investigated by DC tast polarography (DCTP) and differential pulse polarography (DPP), both at a classical dropping mercury electrode (DME), and by differential pulse voltammetry (DPV) and adsorptive stripping differential pulse voltammetry (AdSDPV), both at a miniaturized hanging mercury drop minielectrode (HMDmE), in buffered aqueous (for 5-NQ) or aqueous-methanolic (for 6-NQ and 8-NQ) solutions. Optimum conditions were found for the determination of 5-NQ, 6-NQ and 8-NQ by DCTP at DME (with limits of quantification, LQ ≈ 9 × 10–7, 3 × 10–7 and 2 × 10–6 mol l–1, respectively), by DPP at DME (LQ ≈ 1 × 10–8, 9 × 10–8 and 1 × 10–7 mol l–1, respectively), by DPV at HMDmE (LQ ≈ 2 × 10–8, 1 × 10–7 and 1 × 10–7 mol l–1, respectively), and by AdSDPV at HMDmE (LQ ≈ 1 × 10–8 mol l–1 for 8-NQ; an attempt at increasing the sensitivity using AdSDPV at HMDmE was not successful for 5-NQ and 6-NQ). Practical applicability of the developed methods was verified on the direct determination of the studied compounds in model samples of drinking and river water in submicromolar concentrations and on the determination in model samples of drinking and river water using preliminary separation and preconcentration by solid phase extraction (SPE) in nanomolar concentrations.

Keywords: Analytical methods; Electrochemistry; Polarography; Voltammetry; Mercury electrodes; 5-Nitroquinoline; 6-Nitroquinoline; 8-Nitroquinoline; Spiked water samples; Solid phase extraction.

References: 25 live references.