Collect. Czech. Chem. Commun. 2011, 76, 1811-1823
Published online 2012-01-23 09:40:21

Voltammetric determination of flutamide and its metabolite 4-nitro-3-trifluoromethylaniline at a hanging mercury drop minielectrode

Karolina Peckováa,*, Markéta Průchováa, Josino C. Moreirab, Jiří Bareka, Jan Fischera and Vlastimil Vyskočila

a Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, 128 43 Prague 2, Czech Republic
b CESTEH/ENSP/FIOCRUZ, Rua Leopoldo Bulhoes, 1480 Manguinhos, 21041-210 Rio de Janeiro, Brazil


Optimum conditions were found for direct current and differential pulse voltammetric determination of anticancer drug Flutamide and its main metabolite 4-nitro-3-trifluoromethylaniline on a hanging mercury drop minielectrode in mixed Britton–Robinson buffer (pH 12.0)–methanol (9:1) or 0.01 M NaOH–methanol (9:1) media with limit of quantitation ca. 10–7 mol l–1. It was proved that the newly developed method is applicable for the determination of Flutamide in pharmaceutical formulations and for the determination of both Flutamide and 4-nitro-3-trifluoromethylaniline in urine either directly with limit of quantitation ca. 10–5 mol l–1 or after solid phase extraction with limit of quantitation in the 10–7 mol l–1 concentration range. The sufficient peak potential difference of the two substances suggests the possibility of the analysis of their mixtures.

Keywords: Voltammetry; Analytical methods; Antitumor agents; Flutamide; 4-Nitro-3-trifluoromethylaniline; Hanging mercury drop minielectrode; Solid phase extraction; Urine.

References: 31 live references.