Collect. Czech. Chem. Commun. 2011, 76, 1775-1790
Published online 2012-01-13 10:53:57

Polarographic and voltammetric determination of genotoxic 2-aminofluoren-9-one at mercury electrodes

Andrea Hájkováa, Vlastimil Vyskočila,*, Aleš Daňhela, Joseph Wangb and Jiří Bareka

a Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, 128 43 Prague 2, Czech Republic
b Department of Nanoengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0448 La Jolla, CA, USA


Electrochemical behavior of genotoxic 2-aminofluoren-9-one (2-AFN) was investigated by DC tast polarography (DCTP) and differential pulse polarography (DPP), both at a classical dropping mercury electrode (DME), and by DC voltammetry (DCV), differential pulse voltammetry (DPV), and adsorptive stripping differential pulse voltammetry (AdSDPV), all at a miniaturized hanging mercury drop minielectrode (HMDmE), in buffered aqueous-methanolic solutions. Optimum conditions were found for the determination of 2-AFN by DCTP at DME in the concentration range from 1 × 10–6 to 1 × 10–4 mol l–1 (with a limit of quantification (LQ) of 5 × 10–7 mol l–1), by DPP at DME (from 1 × 10–7 to 1 × 10–4 mol l–1; LQ ≈ 1 × 10–7 mol l–1), by DCV and DPV at HMDmE (both from 1 × 10–7 to 1 × 10–4 mol l–1; LQs ≈ 2 × 10–7 and 1 × 10–7 mol l–1 for DCV and DPV, respectively), and by AdSDPV at HMDmE (from 2 × 10–9 to 1 × 10–7 mol l–1; LQ ≈ 4 × 10–9 mol l–1). Practical applicability of the developed methods was verified on the direct determination of 2-AFN in model samples of drinking and river water in nanomolar to micromolar concentrations.

Keywords: Analytical methods; Electrochemistry; Polarography; Voltammetry; Mercury electrodes; 2-Aminofluoren-9-one; Spiked water samples.

References: 47 live references.