Collect. Czech. Chem. Commun. 2008, 73, 701-704

Preparation of α-5-Aza-2'-deoxy-[6-3H]cytidine

Tomáš Elberta,* and Bohuslav Černýb

a Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic
b Institute of Nuclear Medicine of the First Faculty of Medicine and General Teaching Hospital, Salmovská 3, 120 00 Prague 2, Czech Republic


α-5-Aza-2'-deoxy cytidine was labeled by tritium on the C-6 of the heterocyclic triazine ring. The structure of the α-5-aza-2'-deoxy-[6-3H]cytidine and the position of the label was proved by 3H and 1H NMR. The specific activity was 0.71 TBq mmol-1 (19.2 Ci mmol-1) and radiochemical purity was >99%. The long term stability of the product during the storage at -21 and -72 °C was followed by radio-HPLC.

Keywords: α-5-Aza-2'-deoxy-cytidine; CESG; Tritium; 3H NMR.

References: 7 live references.