Collect. Czech. Chem. Commun. 2008, 73, 187-200
https://doi.org/10.1135/cccc20080187

Determination of Intact Heparin by Capillary Electrophoresis with Contactless Conductivity Detection in Background Electrolytes Containing Hydrophilic Polymers

Petr Tůmaa,*, Eva Samcováa, František Opekarb and Karel Štulíkb

a Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic
b UNESCO Laboratory of Environmental Electrochemistry, Department of Analytical Chemistry, Charles University, Albertov 2030, 128 43 Prague 2, Czech Republic

Abstract

Intact heparin was characterized and determined in model samples, in infusion solutions and in blood plasma by capillary electrophoresis (CE) with contactless conductivity detection. The CE separation of polydisperse heparin took place in open silica capillaries, in electrolytes containing a polymer (hydroxyethyl)cellulose, poly(ethylene glycol) or dextran. The best separation of heparin from excess inorganic ions present in real samples was attained in a background electrolyte consisting of 0.8 M acetic acid and 1% (w/v) dextran (100 kDa). The limit of detection (LOD) was 1.3 μmol l-1. This electrolyte was used in determination of heparin in blood plasma and in infusion solutions.

Keywords: Capillary zone electrophoresis; Contactless conductivity detection; Heparin; Human plasma; Polymers; Polysaccharides; Sulfates.

References: 35 live references.