Collect. Czech. Chem. Commun. 2005, 70, 168-177

FT Raman Spectroscopy as a Tool for Characterization of Derivatized Silica Gel Sorbents

Zuzana Tománková*, Pavel Matějka, Karel Volka and Vladimír Král

Department of Analytical Chemistry, Institute of Chemical Technology, Prague, Technická 5, CZ-166 28 Prague 6, Czech Republic


The potential of Fourier-transform (FT) Raman spectroscopy for verification of individual steps of synthesis of new chromatographic stationary phases was studied. 3-Aminopropylated silica gel was modified with two different optically active compounds ((+)-cloprostenol, (+)-(R)-2,2'-dihydroxy-1,1'-binaphthalene-3-carboxylic acid) via amide bonds. In the next step, free silanol groups were protected with trimethylsilyl groups. The FT Raman spectroscopic results compared with the data obtained by elemental analysis enable not only qualitative verification of synthetic steps, but also a semiquantitative determination of covering of the silica gel surface by derivatization agent.

Keywords: FT Raman spectroscopy; Chiral stationary phases; Sorbents; Surface coverage; Elemental analysis; Binaphthyls; Derivatization of silica gel.

References: 12 live references.