Collect. Czech. Chem. Commun. 2004, 69, 1282-1291

Mild Colorimetric Detection of Sialic Acid

Youjun Yang, Patrick T. Lewis, Jorge O. Escobedo, Nadia N. St. Luce, W. Dale Treleaven, Robert L. Cook and Robert M. Strongin*

Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, U.S.A.


As part of a broader program focusing on the detection of colorless biomolecules in the visible region, a simple and mild procedure for the visual detection of the most abundant sialic acid at room temperature and neutral pH is reported. The role of the interaction of the sialic acid amide with boron in a readily synthesized boronic acid-based receptor is an important feature of the signaling mechanism. Selectivity of reaction can be tuned by the judicious choice of solvents. This study embodies a departure from many of the author's earlier efforts in sugar solutions that are not heated, affording relatively more selective and milder detection.

Keywords: Sialic acids; Uronic acid; Sugars; Saccharides; Resorcinarenes; Calixarenes; Boronates; Boronic acids; Receptors.

References: 20 live references.