Collect. Czech. Chem. Commun. 2004, 69, 322-329

High-Speed Micro-Thermal Focusing Field-Flow Fractionation of Micron-Size Particles

Irina A. Ananievaa,b, Anastasia Yu. Menshikovac, Tatiana G. Evseevac and Josef Jančaa,*

a Université de La Rochelle, Pôle Sciences et Technologie, Avenue Michel Crépeau, 17042 La Rochelle Cedex 01, France
b Lomonosov Moscow State University, Lenin Hills, 119992 Moscow, Russia
c Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoi pr. 31, 199004 Saint Petersburg, Russia


Micron-size polystyrene-based latex particles were separated by using new micro-thermal field-flow fractionation (micro-TFFF). The order of retention from the largest to the smallest particles that appears at high field strength and high flow rate corresponds to the focusing mechanism which itself is a consequence of the lift forces acting on the particles. The mechanism of steric exclusion can only be effective at low flow rates of the carrier liquid. Whenever high-speed separation was performed, the focusing effect clearly dominated the FFF mechanism. This application of micro-TFFF in focusing mode to the separation of the particles is the first one published. As a result, micro-TFFF thus became a very universal technique for the separation of synthetic and natural macromolecules and of particles of various origin and size up to large (micron-size) diameter.

Keywords: Micro-thermal focusing field-flow fractionation; Separation of micron-size particles; Polymers; Macromolecules; Colloids; Polystyrene latex particles.

References: 16 live references.