Collect. Czech. Chem. Commun. 2000, 65, 1055-1066

Investigation of Catechin and Acridine Derivatives Using Voltammetric and Fluorimetric DNA-Based Sensors

Mária Vaníčkováa, Ján Labudaa,*, Miriam Bučkováa, Ioana Surugiub, Michael Mecklenburgb and Bengt Danielssonb

a Department of Analytical Chemistry, Slovak Technical University, SK-81237 Bratislava, Slovak Republic
b Department of Pure and Applied Biochemistry, Lund University, S-221 00 Lund, Sweden


The preconcentration-differential pulse voltammetric detection scheme with a carbon paste electrode bulk phase modified with DNA was used for the investigation of behaviour and the determination of trace levels of catechin and acridine derivatives. The effect of electrochemical activation of the electrode and the DNA-type was examined. The results are compared with those obtained by fluorimetric measurement of the TO-PRO-3 dye : DNA complex in the presence of analytes. With voltammetric biosensors, the detection limits are in the nmol l-1 concentration region. Using competitive reagents, the intercalation of analytes to DNA is indicated.

Keywords: DNA biosensors; DP voltammetry; Fluorescence DNA dye; Catechins; Acridines; Carbon paste electrodes; Electrochemistry.

References: 31 live references.