Collect. Czech. Chem. Commun. 1996, 61, 844-855

Extra-Column Effects in Determination of Rate Parameters by the Chromatographic Method

Olga Šolcová and Petr Schneider

Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, 165 02 Prague 6-Suchdol, Czech Republic


It was shown that the sampling loop, detector and connecting elements in the chromatographic set-up for determination of transport parameters by the dynamic method significantly influence the response peaks from columns packed with porous or nonporous particles. A method, based on the use of convolution theorem, was developed which can take these effects into account. The applicability of this method was demonstrated on the case of axial dispersion in a single-pellet-string column (SPSR) packed with nonporous particles. It is possible to handle also responses from columns packed with porous particles by a similar procedure.

Keywords: Axial dispersion; Peclet number; Single-pellet-string column; Effective diffusion coefficient.