Collect. Czech. Chem. Commun. 1996, 61, 413-422

Extracellular Polysaccharides of Candida mucifera Strain CCY 29-170-1

Alžbeta Kardošová and Vladimír Pätoprstý

Institute of Chemistry, Slovak Academy of Sciences, 842 38 Bratislava, Slovak Republic


Extracellular polysaccharides produced by Candida mucifera strain CCY 29-170-1 in a liquid medium containing malt extract have been investigated by chemical, enzymic, and NMR spectroscopic methods. Mild acid hydrolysis of the native polysaccharide, followed by enzymic treatments of the polymeric residue, afforded two low-molecular neutral homopolysaccharides. The dominant one was a (1→4)-α-D-glucan with approximately each fifth chain unit branched on O-6. The other polysaccharide was a linear (1→4)-β-D-xylan. The results of linkage analysis evidenced that, originally, some xylan-chain units bore on O-3 substituents, which were split off during the hydrolytic steps.

Keywords: Candida mucifera; Extracellular polysaccharides.